Her most recent veterinary check found that she was suffering from a worsening condition, according to the Animal Adventure Park in New York.
Millions of viewers worldwide watched a live stream in 2017 of April giving birth to a calf boy named Tajiri.
Fans of the giraffe tuned in over a seven-week period between February to April in 2017, hoping to catch the birth.
Reacting to the news, one Twitter user said: “I remember this!! I watched the birth and I kept checking on her baby watching it grow too.
“Giraffes are so amazing. She was special. I know she stole a lot of hearts including mine.”
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